Coding

Part:BBa_K1966001

Designed by: Brandon Wilbanks   Group: iGEM16_UT-Knoxville   (2016-10-11)


lacPromoter-xylB

This part contains xylB from Pseudomonas putida under the control of a lac promoter. xylB is benzyl alcohol dehydrogenase (BADH) from Pseudomonas putida. BADH converts a -CH2OH group of benzyl alcohol and its derivatives into -CHOH. This is a basic part that is used in BBa_K1966002 with xylA and xylM (xylene monooxygenase, XO) to generate aromatic aldehydes from toluene derivatives.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 845


[edit]
Categories
Parameters
None